Open AccessCloning, expression, purification, crystallization and preliminary X‐ray analysis of Mycoplasma genitalium protein MG289
Author(s) -
Sippel Katherine H.,
Boehlein Susan K.,
Sakai Yoshihisa,
Quirit Jeanne G.,
AgbandjeMcKenna Mavis,
Rosser Charles J.,
McKenna Robert
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109030565
Subject(s) - mycoplasma genitalium , orthorhombic crystal system , cloning (programming) , urethritis , crystallization , mycoplasma , molecular replacement , biology , crystallography , virology , microbiology and biotechnology , crystal structure , chemistry , chlamydia trachomatis , medicine , computer science , programming language , urology , organic chemistry
Mycoplasma genitalium is a human pathogen that is associated with nongonococcal urethritis in men and cervicitis in women. The cloning, expression, purification and crystallization of the protein MG289 from M. genitalium strain G37 are reported here. Crystals of MG289 diffracted X‐rays to 2.8 Å resolution. The crystals belonged to the orthorhombic space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 49.7, b = 90.9, c = 176.1 Å. The diffraction data after processing had an overall R merge of 8.7%. The crystal structure of Cypl, the ortholog of MG289 from M. hyorhinis , has recently been determined, providing a reasonable phasing model; molecular replacement is currently under way.