Open AccessCrystallization and preliminary X‐ray diffraction analysis of motif N from Saccharomyces cerevisiae Dbf4
Author(s) -
Matthews Lindsay A.,
Duong Andrew,
Prasad Ajai A.,
Duncker Bernard P.,
Guarné Alba
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109029376
Subject(s) - saccharomyces cerevisiae , biology , g2 m dna damage checkpoint , origin recognition complex , dna replication , microbiology and biotechnology , genetics , computational biology , cell cycle , dna , cell cycle checkpoint , eukaryotic dna replication , yeast , gene
The Cdc7–Dbf4 complex plays an instrumental role in the initiation of DNA replication and is a target of replication‐checkpoint responses in Saccharomyces cerevisiae . Cdc7 is a conserved serine/threonine kinase whose activity depends on association with its regulatory subunit, Dbf4. A conserved sequence near the N‐terminus of Dbf4 (motif N) is necessary for the interaction of Cdc7–Dbf4 with the checkpoint kinase Rad53. To understand the role of the Cdc7–Dbf4 complex in checkpoint responses, a fragment of Saccharomyces cerevisiae Dbf4 encompassing motif N was isolated, overproduced and crystallized. A complete native data set was collected at 100 K from crystals that diffracted X‐rays to 2.75 Å resolution and structure determination is currently under way.