Open AccessOverexpression, purification and preliminary X‐ray diffraction analysis of the controller protein C. Csp 231I from Citrobacter sp. RFL231
Author(s) -
Streeter S. D.,
McGeehan J. E.,
Kneale G. G.
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109028681
Subject(s) - escherichia coli , gene , biology , microbiology and biotechnology , chemistry , crystallography , genetics
Restriction–modification controller proteins play an essential role in regulating the temporal expression of restriction–modification genes. The controller protein C. Csp 231I represents a new class of controller proteins. The gene was sublconed to allow overexpression in Escherichia coli . The protein was purified to homogeneity and crystallized using the hanging‐drop vapour‐diffusion method. The crystals diffracted to 2.0 Å resolution and belonged to space group P 2 1 . An electrophoretic mobility‐shift assay provided evidence of strong binding of C. Csp 231I to a sequence located upstream of the csp231IC start codon.