Open AccessPreliminary structural characterization of human SOUL, a haem‐binding protein
Author(s) -
Freire Filipe,
Romão Maria João,
Macedo Anjos L.,
Aveiro Susana S.,
Goodfellow Brian J.,
Carvalho Ana Luísa
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910902291x
Subject(s) - protein data bank (rcsb pdb) , crystallization , crystallography , resolution (logic) , molecule , molecular replacement , chemistry , characterization (materials science) , soul , protein data bank , crystal structure , human liver , protein structure , biochemistry , materials science , in vitro , nanotechnology , computer science , organic chemistry , artificial intelligence , philosophy , theology
Human SOUL (hSOUL) is a 23 kDa haem‐binding protein that was first identified as the PP 23 protein isolated from human full‐term placentas. Here, the overexpression, purification and crystallization of hSOUL are reported. The crystals belonged to space group P 6 4 22, with unit‐cell parameters a = b = 145, c = 60 Å and one protein molecule in the asymmetric unit. X‐ray diffraction data were collected to 3.5 Å resolution at the ESRF. A preliminary model of the three‐dimensional structure of hSOUL was obtained by molecular replacement using the structures of murine p22HBP (PDB codes 2gov and 2hva ), obtained by solution NMR, as search models.