Open AccessPreliminary X‐ray data analysis of crystalline hibiscus chlorotic ringspot virus
Author(s) -
Cheng Ao,
Speir Jeffrey A.,
Yuan Y. Adam,
Johnson John E.,
Wong SekMan
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109015760
Subject(s) - icosahedral symmetry , crystallography , plant virus , capsid , biology , virus , negative stain , resolution (logic) , rna , virology , chemistry , electron microscope , physics , gene , genetics , optics , artificial intelligence , computer science
Hibiscus chlorotic ringspot virus (HCRSV) is a positive‐sense monopartite single‐stranded RNA virus that belongs to the Carmovirus genus of the Tombusviridae family, which includes carnation mottle virus (CarMV). The HCRSV virion has a 30 nm diameter icosahedral capsid with T = 3 quasi‐symmetry containing 180 copies of a 38 kDa coat protein (CP) and encapsidates a full‐length 3.9 kb genomic RNA. Authentic virus was harvested from infected host kenaf leaves and was purified by saturated ammonium sulfate precipitation, sucrose density‐gradient centrifugation and anion‐exchange chromatography. Virus crystals were grown in multiple conditions; one of the crystals diffracted to 3.2 Å resolution and allowed the collection of a partial data set. The crystal belonged to space group R 32, with unit‐cell parameters a = b = 336.4, c = 798.5 Å. Packing considerations and rotation‐function analysis determined that there were three particles per unit cell, all of which have the same orientation and fixed positions, and resulted in tenfold noncrystallography symmetry for real‐space averaging. The crystals used for the structure determination of southern bean mosaic virus (SBMV) have nearly identical characteristics. Together, these findings will greatly aid the high‐resolution structure determination of HCRSV.