Open AccessExpression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9
Author(s) -
Wang Wei,
Wei Lei,
Yang Anqi,
He Teng,
Yuen K. Y.,
Chen Cheng,
Rao Zihe
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109014055
Subject(s) - crystallization , escherichia coli , crystallography , coronavirus , gene , transcription (linguistics) , rna , biology , dna , chemistry , microbiology and biotechnology , virology , covid-19 , genetics , medicine , infectious disease (medical specialty) , linguistics , philosophy , disease , organic chemistry , pathology
Human coronavirus HKU1 (HCoV‐HKU1) belongs to coronavirus group II and encodes 16 nonstructural proteins (nsps) which mediate genome replication and transcription. Among these nsps, nsp9 has been shown to possess single‐stranded DNA/RNA‐binding properties. The gene that encodes HCoV‐HKU1 nsp9 was cloned and expressed in Escherichia coli and the protein was subjected to crystallization trials. The crystals diffracted to 2.7 Å resolution and belonged to space group P 2 1 2 1 2, with unit‐cell parameters a = 83.5, b = 88.4, c = 31.2 Å, α = β = γ = 90° and two molecules per asymmetric unit.