Open AccessPurification, crystallization and preliminary X‐ray diffraction analysis of a plant subtilase
Author(s) -
Rose Rolf,
Huttenlocher Franziska,
Cedzich Anna,
Kaiser Markus,
Schaller Andreas,
Ottmann Christian
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109013979
Subject(s) - crystallization , materials science , diffraction , chemical engineering , optics , physics , engineering
The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting‐drop vapour‐diffusion method. A native data set was collected to 2.5 Å resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl‐derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.