Open AccessProduction, crystallization and preliminary X‐ray diffraction analysis of the allergen Can f 2 from Canis familiaris
Author(s) -
Madhurantakam Chaithanya,
Nilsson Ola B.,
Jönsson Klas,
Grönlund Hans,
Achour Adnane
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109010884
Subject(s) - crystallization , allergen , diffraction , crystallography , crystal (programming language) , resolution (logic) , canis , x ray crystallography , recombinant dna , materials science , chemistry , biology , immunology , optics , allergy , physics , biochemistry , organic chemistry , ecology , artificial intelligence , computer science , gene , programming language
The allergen Can f 2 from dog ( Canis familiaris ) present in saliva, dander and fur is an important cause of allergic sensitization worldwide. Here, the production, isolation, crystallization and preliminary X‐ray diffraction analysis of two crystal forms of recombinant Can f 2 are reported. The first crystal form belonged to space group C 222, with unit‐cell parameters a = 68.7, b = 77.3, c = 65.1 Å, and diffracted to 1.55 Å resolution, while the second crystal form belonged to space group C 2, with unit‐cell parameters a = 75.7, b = 48.3, c = 68.7 Å, β = 126.5°, and diffracted to 2.1 Å resolution. Preliminary data analysis indicated the presence of a single molecule in the asymmetric unit for both crystal forms.