Open AccessStructure of Debaryomyces castellii CBS 2923 phytase
Author(s) -
Ragon M.,
Hoh F.,
Aumelas A.,
Chiche L.,
Moulin G.,
Boze H.
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109008653
Subject(s) - phytase , chemistry , dimer , phosphate , food science , hydrolysis , biochemistry , enzyme , organic chemistry
Phytate ( myo ‐inositol hexakisphosphate) is the primary storage form of phosphate in seeds and legumes (Reddy et al. , 1982). Phytases are phosphatases that hydrolyze phytate to less phosphorylated myo ‐inositol derivatives and inorganic phosphate. The crystal structure of phytase from Debaryomyces castellii has been determined at 2.3 Å resolution. The crystals belonged to space group P 6 5 22, with unit‐cell parameters a = 121.65, c = 332.24 Å. The structure was solved by molecular replacement and refined to a final R factor of 15.7% ( R free = 20.9%). The final model consists of a dimer (with two monomers of 458 residues), five NAG molecules and 628 water molecules.