Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of YvoA from  Bacillus subtilis | Zendy

Resch Marcus | Zendy; Roth Heide Marie | Zendy; Kottmair Mathias | Zendy; Sevvana Madhumati | Zendy; Bertram Ralph | Zendy; Titgemeyer Fritz | Zendy; Muller Yves A. | Zendy

Open Access

Cloning, expression, purification, crystallization and preliminary X‐ray diffraction analysis of YvoA from Bacillus subtilis

Author(s) -

Resch Marcus,

Roth Heide Marie,

Kottmair Mathias,

Sevvana Madhumati,

Bertram Ralph,

Titgemeyer Fritz,

Muller Yves A.

Publication year - 2009

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309109008628

Subject(s) - bacillus subtilis , cloning (programming) , crystallization , diffraction , expression (computer science) , crystallography , computational biology , materials science , biology , chemistry , microbiology and biotechnology , genetics , physics , computer science , optics , bacteria , organic chemistry , programming language

The putative transcriptional regulator protein YvoA (BSU35030) from Bacillus subtilis was cloned and heterologously expressed in Escherichia coli . The protein was purified by immobilized metal‐affinity chromatography and size‐exclusion chromatography and subsequently crystallized. A complete native data set was collected to 2.50 Å resolution. The crystals belonged to the monoclinic space group C 2 and preliminary analysis of the diffraction data indicated the presence of approximately 12 molecules per asymmetric unit.

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