Open AccessOverexpression, purification and crystallization of a thermostable DNA ligase from the archaeon Thermococcus sp. 1519
Author(s) -
Bezsudnova E. Y.,
Kovalchuk M. V.,
Mardanov A. V.,
Poliakov K. M.,
Popov V. O.,
Ravin N. V.,
Skryabin K. G.,
Smagin V. A.,
Stekhanova T. N.,
Tikhonova T. V.
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109007799
Subject(s) - thermococcus , dna ligase , crystallization , chemistry , dna , microbiology and biotechnology , computational biology , biology , archaea , biochemistry , gene , organic chemistry
DNA ligases catalyze the sealing of 5′‐phosphate and 3′‐hydroxyl termini at single‐strand breaks in double‐stranded DNA and their function is essential to maintain the integrity of the genome in DNA metabolism. An ATP‐dependent DNA ligase from the archaeon Thermococcus sp. 1519 was overexpressed, purified and crystallized. Crystals were obtained using the hanging‐drop vapour‐diffusion method employing 35%( v / v ) Tacsimate pH 7.0 as a precipitant and diffracted X‐rays to 3.09 Å resolution. They belonged to space group P 4 1 2 1 2, with unit‐cell parameters a = b = 79.7, c = 182.6 Å.