Open AccessCrystallization and preliminary X‐ray studies of the chromophore‐binding domain of cyanobacteriochrome AnPixJ from Anabaena sp. PCC 7120
Author(s) -
Narikawa Rei,
Muraki Norifumi,
Shiba Tomoo,
Ikeuchi Masahiko,
Kurisu Genji
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108044151
Subject(s) - chromophore , crystallization , x ray , crystallography , anabaena , domain (mathematical analysis) , chemistry , materials science , physics , biology , optics , photochemistry , mathematics , organic chemistry , cyanobacteria , genetics , mathematical analysis , bacteria
Cyanobacteriochromes form a recently defined superfamily of tetrapyrrole‐based photoreceptors that are distantly related to conventional red/far‐red photoreceptor phytochromes. Among these molecules, AnPixJ from Anabaena sp. PCC 7120 is a novel photoreceptor that shows reversible photoconversion between green‐absorbing and red‐absorbing forms, which is in contrast to the properties of conventional phytochromes. In order to better understand the structural basis of this unique photoconversion mechanism, the chromophore‐binding domain of AnPixJ (AnPixJ‐GAF2) was heterologously overproduced and purified, and crystallization of both forms was attempted. Blue crystals of the red‐absorbing form of AnPixJ‐GAF2 were successfully obtained; they belonged to space group P 4 3 2 1 2 and contained one monomer per asymmetric unit. Diffraction data were collected to a resolution of 1.8 Å using synchrotron‐radiation beamline BL‐5A at the Photon Factory.