Open AccessCrystallization and preliminary X‐ray analysis of 2,3‐diketo‐5‐methylthiopentyl‐1‐phosphate enolase from Bacillus subtilis
Author(s) -
Tamura Haruka,
Ashida Hiroki,
Koga Shogo,
Saito Yohtaro,
Yadani Tomonori,
Kai Yasushi,
Inoue Tsuyoshi,
Yokota Akiho,
Matsumura Hiroyoshi
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910804311x
Subject(s) - bacillus subtilis , crystallization , enolase , crystallography , phosphate , materials science , chemistry , biochemistry , biology , medicine , organic chemistry , genetics , bacteria , immunohistochemistry
2,3‐Diketo‐5‐methylthiopentyl‐1‐phosphate enolase (DK‐MTP‐1P enolase) from Bacillus subtilis was crystallized using the hanging‐drop vapour‐diffusion method. Crystals grew using PEG 3350 as the precipitant at 293 K. The crystals diffracted to 2.3 Å resolution at 100 K using synchrotron radiation and were found to belong to the monoclinic space group P 2 1 , with unit‐cell parameters a = 79.3, b = 91.5, c = 107.0 Å, β = 90.8°. The asymmetric unit contained four molecules of DK‐MTP‐1P enolase, with a V M value of 2.2 Å 3 Da −1 and a solvent content of 43%.