Open AccessCrystallization and preliminary X‐ray diffraction analysis of human IL‐22 bound to its soluble decoy receptor IL‐22BP
Author(s) -
Watanabe Leandra,
De Moura Patricia Ribeiro,
Nascimento Alessandro Silva,
Colau Didier,
Dumoutier Laure,
Renauld JeanChristophe,
Polikarpov Igor
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108042309
Subject(s) - decoy , polyethylene glycol , tetragonal crystal system , crystallography , resolution (logic) , crystallization , chemistry , receptor , crystal structure , biochemistry , organic chemistry , artificial intelligence , computer science
Interleukin‐22 (IL‐22) is a pleiotropic cytokine that is involved in inflammatory responses. Human IL‐22 was incubated with its soluble decoy receptor IL‐22BP (IL‐22 binding protein) and the IL‐22–IL‐22BP complex was crystallized in hanging drops using the vapour‐diffusion method. Suitable crystals were obtained from polyethylene glycol solutions and diffraction data were collected to 2.75 Å resolution. The crystal belonged to the tetragonal space group P 4 1 , with unit‐cell parameters a = b = 67.9, c = 172.5 Å, and contained two IL‐22–IL‐22BP complexes per asymmetric unit.