Open AccessCloning, expression, crystallization and preliminary X‐ray crystallographic analysis of glutamyl‐tRNA synthetase (Xoo1504) from Xanthomonas oryzae pv. oryzae
Author(s) -
Doan Thanh Thi Ngoc,
Natarajan Sampath,
Kim Hyesoon,
Ahn YehJin,
Kim JeongGu,
Lee ByoungMoo,
Kang LinWoo
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108039924
Subject(s) - xanthomonas oryzae , xanthomonas oryzae pv. oryzae , crystallization , cloning (programming) , biology , crystallography , genetics , chemistry , gene , organic chemistry , computer science , programming language
The gltX gene from Xanthomonas oryzae pv. oryzae ( Xoo1504 ) encodes glutamyl‐tRNA synthetase (GluRS), one of the most important enzymes involved in bacterial blight (BB), which causes huge production losses of rice worldwide. GluRS is a class I‐type aminoacyl‐tRNA synthetase (aaRS) that is primarily responsible for the glutamylation of tRNA Glu . It plays an essential role in protein synthesis, as well as the regulation of cells, in all organisms. As it represents an important target for the development of new antibacterial drugs against BB, determination of the three‐dimensional structure of GluRS is essential in order to understand its catalytic mechanism. In order to analyze its structure and function, the gltX gene was cloned and the GluRS enzyme was expressed, purified and then crystallized. A GluRS crystal belonging to the monoclinic space group C 2 diffracted to 2.8 Å resolution and had unit‐cell parameters a = 186.8, b = 108.4, c = 166.1 Å, β = 96.3°. The unit‐cell volume of the crystal allowed the presence of six to eight monomers in the asymmetric unit, with a corresponding Matthews coefficient ( V M ) range of 2.70–2.02 Å 3 Da −1 and a solvent‐content range of 54.5–39.3%.