Open AccessCloning, expression, purification, crystallization and preliminary X‐ray crystallographic analysis of isocitrate dehydrogenase 2 (Rv0066c) from Mycobacterium tuberculosis
Author(s) -
Hatzopoulos Georgios N.,
Kefala Georgia,
MuellerDieckmann Jochen
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108035343
Subject(s) - isocitrate dehydrogenase , crystallization , cloning (programming) , mycobacterium tuberculosis , crystallography , chemistry , microbiology and biotechnology , tuberculosis , biology , biochemistry , enzyme , medicine , pathology , organic chemistry , computer science , programming language
Isocitrate dehydrogenase 2 (Icd‐2, Rv0066c) from Mycobacterium tuberculosis was cloned and heterologously expressed in Escherichia coli . The protein was purified by affinity and size‐exclusion chromatography and crystallized. A complete data set has been collected and reduced to 3.25 Å resolution in space group C 2. Preliminary diffraction data analysis suggests a complex packing with at least six molecules in the asymmetric unit.