Open AccessCrystallization and preliminary X‐ray structural studies of adeno‐associated virus serotype 6
Author(s) -
Xie Qing,
Ongley Heather M.,
Hare Joan,
Chapman Michael S.
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108033290
Subject(s) - virology , serotype , crystallization , adeno associated virus , x ray , materials science , crystallography , medicine , biology , physics , chemistry , optics , genetics , gene , vector (molecular biology) , recombinant dna , thermodynamics
Adeno‐associated viruses are being developed as vectors for gene therapy and have been used in a number of clinical trials. Vectors to date have been based on the type species AAV‐2, the structure of which was published in 2002. There is growing interest in modulating the cellular tropism and immune neutralization of AAV‐2 with variants inspired by the properties of other serotypes. Towards the determination of a structure for AAV type 6, this paper reports the high‐yield production, purification, crystallization and preliminary diffraction studies of infectious AAV‐6 virions. The crystals diffracted to 3.2 Å resolution using synchrotron radiation. The most promising crystal form belonged to space group R 3 and appeared to be suitable for initial structure determination.