Open AccessOverproduction, purification and preliminary X‐ray diffraction analysis of YncE, an iron‐regulated Sec‐dependent periplasmic protein from Escherichia coli
Author(s) -
BabaDikwa Aisha,
Thompson Darren,
Spencer Nick J.,
Andrews Simon C.,
Watson Kimberly A.
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108029515
Subject(s) - periplasmic space , escherichia coli , bacterial outer membrane , gene , transport protein , membrane transport protein , chemistry , biology , function (biology) , crystallography , biophysics , membrane protein , biochemistry , transporter , microbiology and biotechnology , membrane
The yncE gene of Escherichia coli encodes a predicted periplasmic protein of unknown function. The gene is de‐repressed under iron restriction through the action of the global iron regulator Fur. This suggests a role in iron acquisition, which is supported by the presence of the adjacent yncD gene encoding a potential TonB‐dependent outer‐membrane transporter. Here, the preliminary crystallographic structure of YncE is reported, revealing that it consists of a seven‐bladed β‐propeller which resembles the corresponding domain of the `surface‐layer protein' of Methanosarcina mazei . A full structure determination is under way in order to provide insight into the function of this protein.