Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ | Zendy

Baranovskiy Andrey G. | Zendy; Babayeva Nigar D. | Zendy; Pavlov Youri I. | Zendy; Tahirov Tahir H. | Zendy

Open Access

Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ

Author(s) -

Baranovskiy Andrey G.,

Babayeva Nigar D.,

Pavlov Youri I.,

Tahirov Tahir H.

Publication year - 2008

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309108025086

Subject(s) - protein subunit , crystallization , crystallography , crystal (programming language) , chemistry , molecule , resolution (logic) , p50 , dna polymerase , crystal structure , dna , stereochemistry , biochemistry , gene , organic chemistry , artificial intelligence , computer science , transcription factor , programming language

Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His‐tagged p50 subunit (p50) and a p50‐interacting domain of the p66 subunit (p66 N ) was crystallized. The crystal was in the form of a prism with a rhombic cross‐section and belonged to space group P 2 1 . The crystal had unit‐cell parameters a = 95.13, b = 248.54, c = 103.46 Å, β = 106.94° and diffracted to a resolution of 3 Å. Four molecules of p50–p66 N in an asymmetric unit corresponded to a crystal solvent content of 72.2%.

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