Open AccessCrystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ
Author(s) -
Baranovskiy Andrey G.,
Babayeva Nigar D.,
Pavlov Youri I.,
Tahirov Tahir H.
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108025086
Subject(s) - protein subunit , crystallization , crystallography , crystal (programming language) , chemistry , molecule , resolution (logic) , p50 , dna polymerase , crystal structure , dna , stereochemistry , biochemistry , gene , organic chemistry , artificial intelligence , computer science , transcription factor , programming language
Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His‐tagged p50 subunit (p50) and a p50‐interacting domain of the p66 subunit (p66 N ) was crystallized. The crystal was in the form of a prism with a rhombic cross‐section and belonged to space group P 2 1 . The crystal had unit‐cell parameters a = 95.13, b = 248.54, c = 103.46 Å, β = 106.94° and diffracted to a resolution of 3 Å. Four molecules of p50–p66 N in an asymmetric unit corresponded to a crystal solvent content of 72.2%.