Open AccessCrystallization and preliminary X‐ray analysis of CrgA, a LysR‐type transcriptional regulator from pathogenic Neisseria meningitidis MC58
Author(s) -
Sainsbury Sarah,
Ren Jingshan,
Saunders Nigel J.,
Stuart David I.,
Owens Raymond J.
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108024068
Subject(s) - biology , neisseria meningitidis , protein data bank (rcsb pdb) , genetics , bacteria , biochemistry
Although LysR‐type regulators (LTTRs) represent the largest family of transcriptional regulators in bacteria, the full‐length structure of only one annotated LTTR (CbnR) has been deposited in the PDB. CrgA, a LTTR from pathogenic Neisseria meningitidis MC58, which is up‐regulated upon bacterial cell contact with human epithelial cells, has been cloned, purified and crystallized. Crystals of full‐length CrgA were obtained after buffer screening with a thermal shift assay and concentration with 0.2 M NDSB‐256. Data were collected from two crystal forms of full‐length CrgA belonging to space groups P 2 1 2 1 2 1 and P 2 1 , diffracting to 3.0 and 3.8 Å resolution and consistent with the presence of between six and ten and between ten and 20 copies of CrgA in the asymmetric unit, respectively. In addition, diffraction data were collected to 2.3 Å resolution from the selenomethionine derivative of the regulatory domain of CrgA. The crystals belonged to space group P 2 1 and contained two molecules in the asymmetric unit.