Open AccessCrystallographic study of G178S mutant of human proliferating cell nuclear antigen
Author(s) -
Hishiki Asami,
Shimizu Toshiyuki,
Serizawa Aya,
Ohmori Haruo,
Sato Mamoru,
Hashimoto Hiroshi
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910802277x
Subject(s) - proliferating cell nuclear antigen , mutant , antigen , microbiology and biotechnology , crystallography , chemistry , biology , genetics , cell growth , gene
Proliferating cell nuclear antigen (PCNA) is an evolutionarily conserved protein that forms a ring‐shaped homotrimer that functions as a sliding clamp for DNA replication. The rev6‐1 mutation of Saccharomyces cerevisiae , which inactivates both translesion DNA synthesis and damage‐avoidance pathways while having little effect on normal cell growth, has a G178S substitution in the PCNA protein. Human PCNA protein carrying the G178S substitution was crystallized. Two crystal forms were obtained under similar conditions. Crystal forms I and II belong to space groups P 2 1 , with unit‐cell parameters a = 84.1, b = 130.2, c = 97.8 Å, β = 113.4°, and P 2 1 2 1 2 1 , with unit‐cell parameters a = 68.1, b = 100.2, c = 131.2 Å, respectively. Structural analyses by molecular replacement are now in progress.