Open AccessExpression, purification, crystallization and preliminary X‐ray analysis of an NADP‐dependent glyceraldehyde‐3‐phosphate dehydrogenase from Helicobacter pylori
Author(s) -
Elliott Paul R.,
Evans Daniel,
Greenwood Jacqueline A.,
Moody Peter C. E.
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108020320
Subject(s) - glyceraldehyde 3 phosphate dehydrogenase , dehydrogenase , nad+ kinase , glyceraldehyde , biochemistry , crystallization , archaea , oxidoreductase , biology , glycolysis , microbiology and biotechnology , helicobacter pylori , enzyme , chemistry , gene , genetics , organic chemistry
The classical glycolytic pathway contains an NAD‐dependent glyceraldehyde‐3‐phosphate dehydrogenase, with NADP‐dependent forms reserved for photosynthetic organisms and archaea. Here, the cloning, expression, purification, crystallization and preliminary X‐ray analysis of an NADP‐dependent glyceraldehyde‐3‐phosphate dehydrogenase from Helicobacter pylori is reported; crystals of the protein were grown both in the presence and the absence of NADP.