Open AccessCrystallization and preliminary X‐ray diffraction analysis of the ternary human GM‐CSF receptor complex
Author(s) -
Hansen Guido,
Hercus Timothy R.,
Xu Yibin,
Lopez Angel F.,
Parker Michael W.,
McKinstry William J.
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108019404
Subject(s) - ternary operation , crystallization , materials science , crystallography , x ray crystallography , x ray , diffraction , chemistry , optics , physics , computer science , organic chemistry , programming language
Granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) is a haemopoietic growth factor that acts though a ternary receptor signalling complex containing specific α (GMRα) and common β (βc) receptor subunits. Human GM‐CSF is encoded by the gene csf2 , while the genes for GMRα and βc are csf2ra and csf2rb , respectively. Crystals of the ternary ectodomain complex comprising GM‐CSF and the soluble extracellular regions of both the GMRα subunit and either βc or its glutamine‐substitution mutant N346Q were obtained using the hanging‐drop vapour‐diffusion method. The best diffracting crystals of the ternary complex were obtained using the N346Q mutation of the βc subunit. These crystals grew using polyethylene glycol 3350 with a high concentration of proline, belonged to space group P 6 3 22 and diffracted to 3.3 Å resolution.