Open AccessCloning, expression and purification of cytochrome c 6 from the brown alga Hizikia fusiformis and complete X‐ray diffraction analysis of the structure
Author(s) -
Akazaki Hideharu,
Kawai Fumihiro,
Chida Hirotaka,
Matsumoto Yuichirou,
Hirayama Mao,
Hoshikawa Ken,
Unzai Satoru,
Hakamata Wataru,
Nishio Toshiyuki,
Park SamYong,
Oku Tadatake
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108017752
Subject(s) - endosymbiosis , biology , eukaryote , chloroplast , cytochrome f , cytochrome c , tetragonal crystal system , crystal structure , crystallography , genome , biochemistry , gene , chemistry , plastid , thylakoid , mitochondrion
The primary sequence of cytochrome c 6 from the brown alga Hizikia fusiformis has been determined by cDNA cloning and the crystal structure has been solved at 1.6 Å resolution. The crystal belonged to the tetragonal space group P 4 1 2 1 2, with unit‐cell parameters a = b = 84.58, c = 232.91 Å and six molecules per asymmetric unit. The genome code, amino‐acid sequence and crystal structure of H. fusiformis cytochrome c 6 were most similar to those of red algal cytochrome c 6 . These results support the hypothesis that brown algae acquired their chloroplasts via secondary endosymbiosis involving a red algal endosymbiont and a eukaryote host.