Open AccessCrystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone
Author(s) -
Morita Hiroyuki,
Tanio Michikazu,
Kondo Shin,
Kato Ryohei,
Wanibuchi Kiyofumi,
Noguchi Hiroshi,
Sugio Shigetoshi,
Abe Ikuro,
Kohno Toshiyuki
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108006775
Subject(s) - atp synthase , crystallization , crystallography , chemistry , polyketide synthase , stereochemistry , polyketide , enzyme , organic chemistry , biosynthesis
Benzalacetone synthase (BAS) from Rheum palmatum is a plant‐specific type III polyketide synthase that catalyzes the one‐step decarboxylative condensation of 4‐coumaroyl‐CoA with malonyl‐CoA to produce the diketide 4‐(4‐hydroxyphenyl)‐but‐3‐en‐2‐one. Recombinant BAS expressed in Escherichia coli was crystallized by the sitting‐drop vapour‐diffusion method. The crystals belong to space group P 2 1 , with unit‐cell parameters a = 54.6, b = 89.6, c = 81.1 Å, α = γ = 90.0, β = 100.5°. Diffraction data were collected to 1.8 Å resolution using synchrotron radiation at BL24XU of SPring‐8.