Open AccessCrystallization and preliminary crystallographic analysis of the second GAF domain of DevS from Mycobacterium smegmatis
Author(s) -
Cho Ha Yeon,
Cho Hyo Je,
Kim Young Min,
Oh Jeong Il,
Kang Beom Sik
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108005186
Subject(s) - mycobacterium smegmatis , crystallization , crystallography , domain (mathematical analysis) , materials science , chemistry , mathematics , organic chemistry , medicine , mycobacterium tuberculosis , tuberculosis , pathology , mathematical analysis
Mycobacterium tuberculosis is known to transform into the nonreplicating persistence state under the influence of hypoxia or nitric oxide. DevS‐DevR is a two‐component regulatory system that mediates the genetic response for the transformation. DevS is a histidine kinase that contains two GAF domains for sensing hypoxia or nitric oxide. The second GAF from M. smegmatis DevS was crystallized using the sitting‐drop vapour‐diffusion method in the presence of sodium citrate and 2‐propanol as precipitants. X‐ray diffraction data were collected from crystals containing selenomethionine to a maximum resolution of 2.0 Å on a synchrotron beamline. The crystals belong to the hexagonal space group P 6 1 . The asymmetric unit contains one molecule, corresponding to a packing density of 2.5 Å 3 Da −1 . The selenium substructure was determined by the single anomalous dispersion method and structure refinement is in progress.