Crystallization and preliminary X‐ray diffraction analysis of the glucuronoyl esterase catalytic domain from Hypocrea jecorina

The catalytic domain of the glucuronoyl esterase from Hypocrea jecorina (anamorph Trichoderma reesei ) was overexpresssed, purified and crystallized by the sitting‐drop vapor‐diffusion method using 1.4  M sodium/potassium phosphate pH 6.9. The crystals belonged to space group P 2 1 2 1 2 1 and X‐ray diffraction data were collected to 1.9 Å resolution. This is the first enzyme with glucoronoyl esterase activity to be crystallized; its structure will be valuable in lignocellulose‐degradation research.