Open AccessCrystallization and preliminary X‐ray crystallographic analysis of rabbit l ‐gulonate 3‐dehydrogenase
Author(s) -
Asada Yukuhiko,
Kuroishi Chizu,
Ukita Yoko,
Sumii Rie,
Endo Satoshi,
Matsunaga Toshiyuki,
Hara Akira,
Kunishima Naoki
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108004326
Subject(s) - monoclinic crystal system , crystallography , crystallization , resolution (logic) , monomer , x ray , synchrotron radiation , diffraction , x ray crystallography , crystal structure , chemistry , crystal (programming language) , solvent , dehydrogenase , materials science , physics , enzyme , optics , polymer , programming language , organic chemistry , artificial intelligence , computer science , biochemistry
Rabbit l ‐gulonate 3‐dehydrogenase was crystallized using the oil‐microbatch method at 295 K. X‐ray diffraction data were collected to 1.70 Å resolution from a crystal at 100 K using synchrotron radiation. The crystal belongs to the C ‐centred monoclinic space group C 2, with unit‐cell parameters a = 71.81, b = 69.08, c = 65.64 Å, β = 102.7°. Assuming the presence of a monomeric protomer in the asymmetric unit gives a V M value of 2.21 Å 3 Da −1 and a solvent content of 44.4%. A cocrystal with NADH, which was isomorphous to the apo form, was also prepared and diffraction data were collected to 1.85 Å resolution using Cu K α radiation at 100 K.