Open AccessCrystallization and preliminary X‐ray diffraction studies of polyketide synthase‐1 (PKS‐1) from Cannabis sativa
Author(s) -
Taguchi Chiho,
Taura Futoshi,
Tamada Taro,
Shoyama Yoshinari,
Shoyama Yukihiro,
Tanaka Hiroyuki,
Kuroki Ryota,
Morimoto Satoshi
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108003795
Subject(s) - polyethylene glycol , polyketide , crystallization , stereochemistry , polyketide synthase , atp synthase , crystallography , chemistry , hepes , crystal structure , biosynthesis , enzyme , organic chemistry , biochemistry
Polyketide synthase‐1 (PKS‐1) is a novel type III polyketide synthase that catalyzes the biosynthesis of hexanoyl triacetic acid lactone in Cannabis sativa (Mexican strain). PKS‐1 was overproduced in Escherichia coli , purified and finally crystallized in two different space groups. The crystal obtained in 0.1 M HEPES buffer pH 7.5 containing 0.2 M calcium acetate and 20%( w / v ) polyethylene glycol 3350 diffracted to 1.65 Å resolution and belonged to space group P 1, with unit‐cell parameters a = 54.3, b = 59.3, c = 62.6 Å, α = 69, β = 81, γ = 80°. Another crystal obtained in 0.1 M HEPES buffer pH 7.5 containing 0.2 M sodium chloride and 20%( w / v ) polyethylene glycol 3350 diffracted to 1.55 Å resolution and belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 54.3, b = 110, c = 130 Å. These data will enable us to determine the crystal structure of PKS‐1.