Open AccessCrystallization and preliminary X‐ray crystallographic analyses of Nur, a nickel‐responsive transcription regulator from Streptomyces coelicolor
Author(s) -
An Young Jun,
Ahn BoEun,
Roe JungHye,
Cha SunShin
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108001760
Subject(s) - streptomyces coelicolor , regulator , crystallization , nickel , transcription (linguistics) , crystallography , x ray , materials science , streptomyces , chemistry , biology , genetics , metallurgy , physics , gene , bacteria , organic chemistry , linguistics , philosophy , quantum mechanics
Nickel ions serve in the correct folding and function of microbial enzymes implicated in metabolic processes. Although nickel ions are indispensable for the survival of cells, the intracellular level of nickel ions needs to be properly maintained as excessive levels of nickel ions are toxic. Nur, a nickel‐uptake regulator belonging to the Fur family, is a nickel‐responsive transcription factor that controls nickel homeostasis and antioxidative response in Streptomyces coelicolor . Nur was purified and crystallized at 295 K. A 2.4 Å native data set and a 3.0 Å Ni‐MAD data set were collected using synchrotron radiation. The Nur crystals belong to space group P 3 1 , with unit‐cell parameters a = b = 78.17, c = 50.39 Å. Assuming the presence of two molecules in the asymmetric unit, the solvent content is estimated to be about 54.7%.