Open AccessProduction, purification and preliminary X‐ray crystallographic studies of adeno‐associated virus serotype 7
Author(s) -
Quesada Odayme,
Gurda Brittney,
Govindasamy Lakshmanan,
McKenna Robert,
Kohlbrenner Erik,
Aslanidi George,
Zolotukhin Sergei,
Muzyczka Nicholas,
AgbandjeMcKenna Mavis
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107060289
Subject(s) - capsid , icosahedral symmetry , crystallography , diffraction , x ray , x ray crystallography , crystal structure , resolution (logic) , materials science , virus , virology , chemistry , physics , biology , optics , artificial intelligence , computer science
Crystals of baculovirus‐expressed adeno‐associated virus serotype 7 capsids diffract X‐rays to ∼3.0 Å resolution. The crystals belong to the rhombohedral space group R 3, with unit‐cell parameters a = 252.4, c = 591.2 Å in the hexagonal setting. The diffraction data were processed and reduced to an overall completeness of 79.0% and an R merge of 12.0%. There are three viral capsids in the unit cell. The icosahedral threefold axis is coincident with the crystallographic threefold axis, resulting in one third of a capsid (20 monomers) per crystallographic asymmetric unit. The orientation of the viral capsid has been determined by rotation‐function searches and is positioned at (0, 0, 0) by packing considerations.