Open AccessCrystallization and preliminary X‐ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin
Author(s) -
Nakamura Toshio,
Tonozuka Takashi,
Kotani Mao,
Obata Kanae,
Oguma Keiji,
Nishikawa Atsushi
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107053973
Subject(s) - toxin , clostridium botulinum , crystallization , chemistry , recombinant dna , escherichia coli , microbiology and biotechnology , molecule , crystallography , stereochemistry , biochemistry , biology , organic chemistry , gene
HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli , purified and crystallized. Diffraction data were collected to 2.6 Å resolution and the crystal belonged to the hexagonal space group P 6 3 . Matthews coefficient and self‐rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy‐atom derivatives has been undertaken.