Open AccessPurification, crystallization and preliminary X‐ray diffraction analysis of water‐soluble chlorophyll‐binding protein from Chenopodium album
Author(s) -
Ohtsuki Takayuki,
Ohshima Shigeru,
Uchida Akira
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107035658
Subject(s) - chenopodium , crystallization , crystallography , chlorophyll , botany , chlorophyll a , chenopodiaceae , chemistry , chromatography , biology , organic chemistry , weed
A water‐soluble chlorophyll‐binding protein (WSCP) with photoconvertibility from Chenopodium album was extracted, purified and crystallized in a darkroom. Green crystals suitable for data collection appeared in about 10 d. A native data set was collected to 2.0 Å resolution at 100 K. The space group of the crystal was determined to be orthorhombic I 222 or I 2 1 2 1 2 1 , with unit‐cell parameters a = 48.13, b = 60.59, c = 107.21 Å. Preliminary analysis of the X‐ray data indicated that there is one molecule per asymmetric unit.