Open AccessPreliminary X‐ray analysis of cellobiohydrolase Cel7B from Melanocarpus albomyces
Author(s) -
Parkkinen Tarja,
Koivula Anu,
Vehmaanperä Jari,
Rouvinen Juha
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107034458
Subject(s) - cellobiose , crystallography , glycoside hydrolase , chemistry , cellulose , crystal twinning , hydrolysis , materials science , cellulase , biochemistry , microstructure
Cellobiohydrolases are enzymes that cleave off cellobiose units from cellulose chains in a processive manner. Melanocarpus albomyces Cel7B is a thermostable single‐module cellobiohydrolase that has relatively low activity on small soluble substrates at room temperature. It belongs to glycoside hydrolase family 7, which includes endo‐β‐1,4‐glucanases and cellobiohydrolases. Cel7B was crystallized using the hanging‐drop vapour‐diffusion method and streak‐seeding. The crystals belonged to space group P 2 1 , with unit‐cell parameters a = 50.9, b = 94.5, c = 189.8 Å, β = 90.0° and four monomers in the asymmetric unit. Analysis of the intensity statistics showed that the crystals were pseudo‐merohedrally twinned, with a twinning fraction of 0.37. X‐ray diffraction data were collected at 1.6 Å resolution using synchrotron radiation.