Open AccessCrystallization and preliminary X‐ray diffraction analysis of the cytosolic domain of the Mg 2+ transporter MgtE
Author(s) -
Tanaka Yoshiki,
Hattori Motoyuki,
Fukai Shuya,
Ishitania Ryuichiro,
Nureki Osamu
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107032344
Subject(s) - crystallization , x ray crystallography , crystallography , diffraction , domain (mathematical analysis) , cytosol , transporter , materials science , chemistry , physics , optics , biochemistry , gene , organic chemistry , enzyme , mathematical analysis , mathematics
The MgtE family of Mg 2+ transporters is ubiquitously conserved in all domains of life. The cytosolic domains of the MgtE Mg 2+ transporters include a cystathionine‐β‐synthase (CBS) domain which is known to play a regulatory function in transporter proteins. The cytosolic domain of MgtE from Thermus thermophilus was overexpressed, purified and crystallized in the presence and absence of Mg 2+ . The crystals formed in the presence of Mg 2+ diffracted X‐rays to 2.3 Å resolution using synchrotron radiation, belong to space group P 6 5 22 with unit‐cell parameters a = b = 57.7, c = 317.6 Å and are expected to contain one molecule in the asymmetric unit. The crystals formed in the absence of Mg 2+ diffracted X‐rays to 3.5 Å resolution using synchrotron radiation, belong to space group P 2 1 2 1 2 1 with unit‐cell parameters a = 77.0, b = 100.3, c = 100.3 Å and are expected to contain two molecules in the asymmetric unit.