Open AccessCrystallization and preliminary X‐ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins
Author(s) -
Shirokane Michio,
Sawano Yoriko,
Miyazono Kenichi,
Nagata Koji,
Tanokura Masaru
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107024487
Subject(s) - pyrococcus horikoshii , crystallization , materials science , crystallography , chemistry , crystal structure , organic chemistry
PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins, was expressed, purified and crystallized. Crystallization was performed by the sitting‐drop vapour‐diffusion method using PEG 3350 as the precipitant. The crystal diffracted X‐rays to 1.90 Å resolution using a synchrotron‐radiation source. The space group of the crystal was determined to be P 2 1 2 1 2 1 , with unit‐cell parameters a = 46.9, b = 49.5, c = 132.7 Å. The crystal contained two PH1010 molecules in the asymmetric unit ( V M = 2.4 Å 3 Da −1 ) and had a solvent content of 48%.