Open AccessCrystallization and preliminary X‐ray analysis of the electron‐transfer complex of Rieske‐type [2Fe–2S] ferredoxin and NADH‐dependent ferredoxin reductase derived from Acidovorax sp. strain KKS102
Author(s) -
Senda Miki,
Kishigami Shinya,
Kimura Shigenobu,
Senda Toshiya
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107023007
Subject(s) - ferredoxin , electron transfer , crystallography , crystallization , reductase , strain (injury) , electron transport chain , oxidoreductase , materials science , chemistry , biology , photochemistry , biochemistry , enzyme , organic chemistry , anatomy
The electron‐transfer complex of BphA3, a Rieske‐type [2Fe–2S] ferredoxin, and BphA4, a NADH‐dependent ferredoxin reductase, was crystallized using the sitting‐drop vapour‐diffusion method under anaerobic conditions. The obtained crystals were analyzed by SDS–PAGE, which showed that they contained both BphA3 and BphA4. The crystals belong to space group P 2 1 , with unit‐cell parameters a = 60.60, b = 173.72, c = 60.98 Å, β = 115.8°, and diffracted to a resolution of 1.9 Å.