Open AccessCrystallization and preliminary X‐ray analysis of the reduced Rieske‐type [2Fe–2S] ferredoxin derived from Pseudomonas sp. strain KKS102
Author(s) -
Senda Miki,
Kimura Shigenobu,
Kishigami Shinya,
Senda Toshiya
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107009992
Subject(s) - ferredoxin , strain (injury) , biphenyl , crystallization , dioxygenase , crystallography , pseudomonas , chemistry , materials science , stereochemistry , bacteria , biology , organic chemistry , enzyme , genetics , anatomy
The reduced form of BphA3, a Rieske‐type [2Fe–2S] ferredoxin component of the biphenyl dioxygenase BphA from Pseudomonas sp. strain KKS102, was crystallized by the sitting‐drop vapour‐diffusion method under anaerobic conditions. The crystal belongs to space group P 3 1 21, with unit‐cell parameters a = b = 49.6, c = 171.9 Å, and diffracts to a resolution of 1.95 Å. A molecular‐replacement calculation using oxidized BphA3 as a search model yielded a satisfactory solution.