Open AccessPreparation and preliminary X‐ray diffraction analysis of crystals of bacterial flagellar sigma factor σ 28 in complex with the σ 28 ‐binding region of its antisigma factor, FlgM
Author(s) -
Okada Kengo,
Ichihara Hisako,
Takahashi Hiroyuki,
Fujita Nobuyuki,
Ishihama Akira,
Hakoshima Toshio
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910700509x
Subject(s) - sigma factor , escherichia coli , transcription factor , crystallography , flagellum , biology , x ray crystallography , transcription (linguistics) , resolution (logic) , biophysics , chemistry , microbiology and biotechnology , gene , diffraction , physics , biochemistry , rna polymerase , optics , linguistics , philosophy , artificial intelligence , computer science
The sigma 28 kDa (σ 28 ) factor is a transcription factor specific for the expression of bacterial flagellar and chemotaxis genes. Its antisigma factor, FlgM, binds σ 28 factor and inhibits its activity as a transcription factor. In this study, crystals of the complex between Escherichia coli σ 28 and the C‐terminal σ 28 ‐binding region of FlgM were obtained. The crystals belong to space group P 3 1 21 or P 3 2 21, with unit‐cell parameters a = b = 106.7 (2), c = 51.74 (3) Å, containing one complex in the crystallographic asymmetric unit. An X‐ray intensity data set was collected to a resolution of 2.7 Å.