Open AccessCloning, expression, purification, crystallization and preliminary X‐ray crystallographic study of DHNA synthetase from Geobacillus kaustophilus
Author(s) -
Kanaujia Shankar Prasad,
Ranjani Chellamuthu Vasuki,
Jeyakanthan Jeyaraman,
Baba Seiki,
Kuroishi Chizu,
Ebihara Akio,
Shinkai Akeo,
Kuramitsu Seiki,
Shiro Yoshitsugu,
Sekar Kanagaraj,
Yokoyama Shigeyuki
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106056521
Subject(s) - cloning (programming) , crystallization , crystallography , geobacillus stearothermophilus , chemistry , thermophile , biochemistry , microbiology and biotechnology , biology , enzyme , organic chemistry , computer science , programming language
The aerobic Gram‐positive bacterium Geobacillus kaustophilus is a bacillus species that was isolated from deep‐sea sediment from the Mariana Trench. 1,4‐Dihydroxy‐2‐naphthoate (DHNA) synthetase plays a vital role in the biosynthesis of menaquinone (vitamin K 2 ) in this bacterium. DHNA synthetase from Geobacillus kaustophilus was crystallized in the orthorhombic space group C 222 1 , with unit‐cell parameters a = 77.01, b = 130.66, c = 131.69 Å. The crystal diffracted to a resolution of 2.2 Å. Preliminary studies and molecular‐replacement calculations reveal the presence of three monomers in the asymmetric unit.