Open AccessExploration of triple‐helical fragments: crystallization and preliminary X‐ray diffraction of d(TGGCCTTAAGG)
Author(s) -
Van Hecke Kristof,
Uytterhoeven Koen,
Van Meervelt Luc
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106054522
Subject(s) - orthorhombic crystal system , crystallography , diffraction , crystallization , crystal structure , thymine , x ray crystallography , resolution (logic) , chemistry , high resolution , materials science , physics , optics , dna , geology , biochemistry , remote sensing , organic chemistry , artificial intelligence , computer science
The nonamer d(GCGAATTCG) and decamer d(GGCCAATTGG), containing one and two overhanging guanines, respectively, form G·GC triplets in their crystal packing. In order to introduce a third subsequent T·AT triplet, the decamer was further extended by one overhanging thymine residue. Two different crystal morphologies of the sequence d(TGGCCTTAAGG) were obtained by hanging‐drop vapour diffusion and diffracted to 2.5 and 2.3 Å resolution, respectively. However, both crystals belong to the orthorhombic space group P 2 1 2 1 2 1 , with similar unit‐cell parameters. Therefore, the two data sets could be merged to a resolution of 2.4 Å with unit‐cell parameters a = 26.97, b = 41.12, c = 52.72 Å.