Crystallization of ornithine acetyltransferase from yeast by counter‐diffusion and preliminary X‐ray study | Zendy

Maes Dominique | Zendy; Crabeel Marjolaine | Zendy; Van de Weerdt Cécile | Zendy; Martial Joseph | Zendy; Peeters Eveline | Zendy; Charlier Daniël | Zendy; Decanniere Klaas | Zendy; Vanhee Celine | Zendy; Wyns Lode | Zendy; Zegers Ingrid | Zendy

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Crystallization of ornithine acetyltransferase from yeast by counter‐diffusion and preliminary X‐ray study

Author(s) -

Maes Dominique,

Crabeel Marjolaine,

Van de Weerdt Cécile,

Martial Joseph,

Peeters Eveline,

Charlier Daniël,

Decanniere Klaas,

Vanhee Celine,

Wyns Lode,

Zegers Ingrid

Publication year - 2006

Publication title -

acta crystallographica section f

Language(s) - English

Resource type - Journals

ISSN - 1744-3091

DOI - 10.1107/s1744309106050561

Subject(s) - crystallization , yeast , crystallography , diffusion , arginine , chemistry , acetyltransferase , materials science , biochemistry , amino acid , physics , organic chemistry , thermodynamics , acetylation , gene

A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter‐diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging‐drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P 4, with unit‐cell parameters a = b = 66.98, c = 427.09 Å, and a data set was collected to 2.76 Å.

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