Open AccessCloning, expression, purification, crystallization and preliminary crystallographic analysis of pseudo death‐effector domain of HIPPI, a molecular partner of Huntingtin‐interacting protein HIP‐1
Author(s) -
Banerjee Manisha,
Majumder Pritha,
Bhattacharyya Nitai P.,
Dattagupta Jiban K.,
Sen Udayaditya
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106046628
Subject(s) - effector , apoptosis , interactor , crystallization , huntingtin , crystallography , cloning (programming) , chemistry , microbiology and biotechnology , molecular cloning , affinity chromatography , gene , biology , biochemistry , gene expression , computer science , mutant , organic chemistry , programming language , enzyme
The formation of a heterodimer between Huntingtin‐interacting protein‐1 (HIP‐1) and its novel partner HIPPI (HIP‐1 protein interactor) through their pseudo death‐effector domains (pDEDs) is a key step that recruits caspase‐8 and initiates apoptosis. This could be one of the pathways by which apoptosis is increased in Huntington's disease (HD). A construct consisting of the pDED of HIPPI has been cloned and overexpressed as 6NH‐tagged protein and purified by Ni–NTA affinity chromatography. Crystals of the pDED of HIPPI were grown in space group P 4 1 , with unit‐cell parameters a = b = 77.42, c = 33.31 Å and a calculated Matthews coefficient of 1.88 Å 3 Da −1 (33% solvent content) with two molecules per asymmetric unit.