Open AccessCrystallization and preliminary X‐ray analysis of a protease inhibitor from the latex of Carica papaya
Author(s) -
Azarkan Mohamed,
GarciaPino Abel,
Dibiani Rachid,
Wyns Lode,
Loris Remy,
BaeyensVolant Danielle
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106046367
Subject(s) - crystallization , divalent , carica , crystallography , resolution (logic) , crystal (programming language) , chemistry , molecule , crystal structure , stereochemistry , materials science , organic chemistry , botany , biology , artificial intelligence , computer science , programming language
A Kunitz‐type protease inhibitor purified from the latex of green papaya ( Carica papaya ) fruits was crystallized in the presence and absence of divalent metal ions. Crystal form I, which is devoid of divalent cations, diffracts to a resolution of 2.6 Å and belongs to space group P 3 1 or P 3 2 . This crystal form is a merohedral twin with two molecules in the asymmetric unit and unit‐cell parameters a = b = 74.70, c = 78.97 Å. Crystal form II, which was grown in the presence of Co 2+ , diffracts to a resolution of 1.7 Å and belongs to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 44.26, b = 81.99, c = 140.89 Å.