Open AccessExpression, purification, crystallization and preliminary X‐ray diffraction analysis of mitochondrial thioredoxin Trx3 from Saccharomyces cerevisiae
Author(s) -
Bao Rui,
Chen Yuxing,
Zhang Yaru,
Zhou CongZhao
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106041467
Subject(s) - saccharomyces cerevisiae , thioredoxin , crystallization , yeast , escherichia coli , crystallography , biochemistry , crystal (programming language) , chemistry , biology , enzyme , gene , organic chemistry , computer science , programming language
There are three thioredoxin isoforms in the yeast Saccharomyces cerevisiae : two cytosolic/nuclear thioredoxins, Trx1 and Trx2, and one mitochondrial thioredoxin, Trx3. In the present work, S. cerevisiae Trx3 overexpressed in Escherichia coli was purified and crystallized. The Trx3 crystals were obtained by the hanging‐drop vapour‐diffusion method. A data set diffracting to 2.0 Å resolution was collected from a single crystal. The crystal belongs to space group P 3 1 , with unit‐cell parameters a = b = 49.57, c = 94.55 Å, α = β = 90, γ = 120°. The asymmetric unit is assumed to contain two subunits of Trx3, with a V M value of 2.62 Å 3 Da −1 and a solvent content of 53%.