Open AccessCrystallization of Ranasmurfin, a blue‐coloured protein from Polypedates leucomystax
Author(s) -
McMahon Stephen A.,
Walsh Martin A.,
Ching Rosalind Tan Yan,
Carter Lester G.,
Dorward Mark,
Johnson Kenneth A.,
Liu Huanting,
Oke Muse,
Bloch Carlos,
Kennedy Malcolm W.,
Latiff Aishah A.,
Cooper Alan,
Taylor Garry L.,
White Malcolm F.,
Naismith James H.
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106040036
Subject(s) - dimer , crystallization , crystallography , fluorescence , chemistry , protein crystallization , biophysics , biology , physics , optics , organic chemistry
Ranasmurfin, a previously uncharacterized ∼13 kDa blue protein found in the nests of the frog Polypedates leucomystax , has been purified and crystallized. The crystals are an intense blue colour and diffract to 1.51 Å with P 2 1 symmetry and unit‐cell parameters a = 40.9, b = 59.9, c = 45.0 Å, β = 93.3°. Self‐rotation function analysis indicates the presence of a dimer in the asymmetric unit. Biochemical data suggest that the blue colour of the protein is related to dimer formation. Sequence data for the protein are incomplete, but thus far have identified no model for molecular replacement. A fluorescence scan shows a peak at 9.676 keV, indicating that the protein binds zinc and suggesting a route for structure solution.