Open AccessCloning, expression, purification, crystallization and preliminary structure determination of glucose‐1‐phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose‐1‐phosphate
Author(s) -
Aragão D.,
Marques A. R.,
Frazão C.,
Enguita F. J.,
Carrondo M. A.,
Fialho A. M.,
SáCorreia I.,
Mitchell E. P.
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106030107
Subject(s) - crystallization , phosphate , crystal (programming language) , derivative (finance) , crystallography , cloning (programming) , chemistry , biochemistry , organic chemistry , computer science , financial economics , economics , programming language
The cloning, expression, purification, crystallization and preliminary crystallographic analysis of glucose‐1‐phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose‐1‐phosphate are reported. Diffraction data sets were obtained from seven crystal forms in five different space groups, with highest resolutions ranging from 4.20 to 2.65 Å. The phase problem was solved for a P 2 1 crystal form using multiple isomorphous replacement with anomalous scattering from an osmium derivative and a SeMet derivative. The best native crystal in space group P 2 1 has unit‐cell parameters a = 105.5, b = 85.7, c = 151.8 Å, β = 105.2°. Model building and refinement are currently under way.