Open AccessCross‐crystallization method used for the crystallization and preliminary diffraction analysis of a novel di‐haem cytochrome c 4
Author(s) -
Branca Rui Miguel Mamede,
Bodó Gabriella,
Bagyinka Csaba,
Tomčová Ivana,
Kutá Smatanová Ivana
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106027710
Subject(s) - crystallization , crystallography , cytochrome , tetragonal crystal system , chemistry , cytochrome c , sulfur , x ray crystallography , diffraction , analytical chemistry (journal) , materials science , physics , crystal structure , optics , biochemistry , chromatography , organic chemistry , enzyme , mitochondrion
The newly discovered di‐haem cytochrome c 4 from the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina is the first cytochrome c 4 to be crystallized from an anaerobic organism. It was crystallized using the addition of metal‐ion salts to the standard vapour‐diffusion method. Coloured well shaped three‐dimensional crystals with dimensions of approximately 0.6 × 0.05 × 0.02 mm grew within 3–4 d at pH 5 and diffracted to 1.72 Å without radiation damage. Cytochrome c 4 crystallized in space group P 4 1 2 1 2 as a primitive tetragonal system with unit‐cell parameters a = b = 75.29, c = 37.12 Å, α = β = γ = 90°.