Crystallization and preliminary crystallographic analysis of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7

Crystallization and preliminary crystallographic studies of type 1 RNase H from the hyperthermophilic archaeon Sulfolobus tokodaii 7 were performed. A crystal was grown at 277 K by the sitting‐drop vapour‐diffusion method. Native X‐ray diffraction data were collected to 1.5 Å resolution using synchrotron radiation from station BL41XU at SPring‐8. The crystal belongs to space group P 4 3 , with unit‐cell parameters a = b = 39.21, c = 91.15 Å. Assuming the presence of one molecule in the asymmetric unit, the Matthews coefficient V M was calculated to be 2.1 Å 3  Da −1 and the solvent content was 40.5%. The structure of a selenomethionine Sto‐RNase HI mutant obtained using a MAD data set is currently being analysed.