Open AccessCrystallization, data collection and phasing of two digestive lysozymes from Musca domestica
Author(s) -
Marana S. R.,
Terra W. R.,
Cançado F. C.,
Ferreira C.,
Valério A. A.,
Barbosa J. A. R. G.
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309106024201
Subject(s) - musca , digestive tract , biology , zoology , ecology , medicine , larva
Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting‐drop vapour‐diffusion method in the presence of ammonium sulfate or PEG/2‐propanol as the precipitant. X‐ray diffraction data were collected to a maximum resolution of 1.9 Å using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P 2 1 (unit‐cell parameters a = 36.52, b = 79.44, c = 45.20 Å, β = 102.97°) and the orthorhombic space group P 2 1 2 1 2 (unit‐cell parameters a = 73.90, b = 96.40, c = 33.27 Å), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress.